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Table 1 A citation record that shows the MEDLINE fields. Fukagawa T, Nogami M, Yoshikawa M, Ikeno M, Okazaki T, Takami Y, Nakayama T, Oshimura M. Dicer is essential for formation of the heterochromatin structure in vertebrate cells. Nat Cell Biol. 2004 Aug;6(8):784-91.

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PMID 15247924
DA 20040810
DCOM 20040827
LR 20051122
PUBM Print-Electronic
IS 1465–7392 (Print)
VI 6
IP 8
DP 2004 Aug
TI Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
PG 784-91
AB RNA interference is an evolutionarily conserved gene-silencing pathway in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. The biological function of the RNAi-related pathway in vertebrate cells is not fully understood. Here, we report the generation of a conditional loss-of-function Dicer mutant in a chicken-human hybrid DT40 cell line that contains human chromosome 21. We show that loss of Dicer results in cell death with the accumulation of abnormal mitotic cells that show premature sister chromatid separation. Aberrant accumulation of transcripts from alpha-satellite sequences, which consist of human centromeric repeat DNAs, was detected in Dicer-deficient cells. Immunocytochemical analysis revealed abnormalities in the localization of two heterochromatin proteins, Rad21 cohesin protein and BubR1 checkpoint protein, but the localization of core kinetochore proteins such as centromere protein (CENP)-A and -C was normal. We conclude that Dicer-related RNA interference machinery is involved in the formation of the heterochromatin structure in higher vertebrate cells.
AD Precursory Research for Embryonic Science and Technology of Japan Science and Technology Agency, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan.
FAU Fukagawa, Tatsuo
AU Fukagawa T
FAU Nogami, Masahiro
AU Nogami M
FAU Yoshikawa, Mitsuko
AU Yoshikawa M
FAU Ikeno, Masashi
AU Ikeno M
FAU Okazaki, Tuneko
AU Okazaki T
FAU Takami, Yasunari
AU Takami Y
FAU Nakayama, Tatsuo
AU Nakayama T
FAU Oshimura, Mitsuo
AU Oshimura M
LA eng
PT Journal Article
DEP 20040711
PL England
TA Nat Cell Biol
JT Nature cell biology.
JID 100890575
RN 0 (Cell Cycle Proteins)
RN 0 (Heterochromatin)
RN 0 (Nuclear Proteins)
RN 0 (Phosphoproteins)
RN 0 (RAD21 protein, human)
RN EC 2.7.1.- (Bub1 spindle checkpoint protein)
RN EC (Protein Kinases)
RN EC 3.1.- (Endoribonucleases)
CIN Nat Cell Biol. 2004 Aug;6(8):696-7. PMID: 15303098
MH Animals
MH Blotting, Western
MH Cell Cycle Proteins/genetics/metabolism
MH Cell Death/genetics
MH Cell Line
MH Cell Survival
MH Centromere/chemistry
MH Chickens
MH Chromosomes, Human, Pair 21
MH Endoribonucleases/deficiency/*genetics/*physiology
MH Gene Silencing
MH Heterochromatin/*chemistry/genetics/*metabolism
MH Humans
MH Immunohistochemistry
MH In Situ Hybridization, Fluorescence
MH Models, Biological
MH Mutation
MH Nuclear Proteins/genetics/metabolism
MH Phosphoproteins/genetics/metabolism
MH Protein Kinases/genetics/metabolism
MH RNA Interference
MH Research Support, Non-U.S. Gov't
MH Restriction Mapping
MH Transgenes
EDAT 7/13/2004 5:00
MHDA 8/28/2004 5:00
PHST 2004/05/29 [received]
PHST 2004/06/29 [accepted]
PHST 2004/07/11 [aheadofprint]
AID 10.1038/ncb1155 [doi]
AID ncb1155 [pii]
PST ppublish
SO Nat Cell Biol. 2004 Aug;6(8):784-91. Epub 2004 Jul 11.